Taxonomic assignment report

Facility	Hogwarts
Analyst	John Doe
Analysis started	2025-04-06 06:38:33
Analysis completed	2025-04-06 06:38:33
Wall time	0:0:0 hours

Sample details

locus	COI
preliminary_id	Aphididae
taxa_of_interest	Aphis spiraecola Aphididae
country	Netherlands
host	Cut flower Paenonia
sample_id	VE24-1066_COI
Query DNA sequence

Result overview

Inconclusive
The analyst should attempt subjective species identification at the genus level.
Reasoning - Flag 1C: >3 candidate species matched with high stringency (identity ≥ 98.5%).

Preliminary morphology ID confirmed	NA
Inconclusive taxonomic identity (Flag 1C)

Taxa of interest ruled out	False
Flag 2B: Taxon of interest detected Flag 5.1NA: Assessment of related species is only possible for taxa at rank genus/species Flag 5.2NA: Assessment of related species is only possible for taxa at rank genus/species

Analyst evaluation

Identification of candidate species

Flag 1C: The analyst should attempt subjective species identification at the genus level
>3 candidate species matched with high stringency (identity ≥ 98.5%)

Candidate hits must meet ONE of these criteria:

Minimum alignment length	`400bp`
Minimum query coverage	`85.0%`

Candidate hits are then classified as follows:

Classification	Alignment identity	Number of hits	Number of species
STRONG MATCH	≥ 98.5%	292	6
MODERATE MATCH	≥ 93.5%	NA	NA
NO MATCH	< 93.5%

Hits per candidate species (top 10 candidates only)

Species	Hits	Identity
Aphis spiraecola	273	99.8%
Aphis fabae	4	99.8%
Aphis pomi	9	99.8%
Aphis sp. BOLD:AAA4183	3	99.8%
Aphididae sp. BOLD:AAA4183	2	99.8%
Aphis gossypii	1	99.5%

#	Accession	Hit subject	Align length	Query coverage	Score	E-value	Identity
1	MT445566	Aphis spiraecola isolate Pometum (DK) cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
2	AB506735	Aphis spiraecola mitochondrial COI gene for cytochrome oxidase subunit I, partial cds, isolate: Aspi1	658	100.0%	655.0	0.00e+00	99.8%
3	MN319941	Aphis spiraecola voucher NIBGE APH-00631 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
4	MN320240	Aphis spiraecola voucher NIBGE APH-00321 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
5	MW315438	Aphis spiraecola voucher A34 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
6	PQ157613	Aphis spiraecola isolate CITHAs4 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
7	KY837424	Aphis spiraecola voucher BIOUG02527-A03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
8	MN319946	Aphis spiraecola voucher NIBGE APH-00696 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
9	KY844604	Aphis spiraecola voucher BIOUG02527-A05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
10	PQ164396	Aphis spiraecola isolate CITHAs5 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
11	KY842196	Aphis spiraecola voucher BIOUG02439-G09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
12	MN320096	Aphis spiraecola voucher NIBGE APH-00587 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
13	KY835246	Aphis spiraecola voucher BIOUG02439-H02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
14	MH632734	Aphis spiraecola isolate AsPOIV cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
15	MW315443	Aphis spiraecola voucher A39 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
16	PQ157565	Aphis spiraecola isolate CITHAs1 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
17	KY842009	Aphis spiraecola voucher BIOUG02527-B07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
18	MN320226	Aphis spiraecola voucher NIBGE APH-00643 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
19	NC_053819	Aphis spiraecola mitochondrion, complete genome	658	100.0%	655.0	0.00e+00	99.8%
20	KY836697	Aphis spiraecola voucher BIOUG02527-E09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
21	MN320191	Aphis spiraecola voucher NIBGE APH-00649 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
22	KY000675	Aphis spiraecola voucher Apspir-2016 cytochrome c oxidase subunit 1 (COX1) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
23	MW315446	Aphis spiraecola voucher A42 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
24	KY833484	Aphis spiraecola voucher BIOUG02527-C02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
25	MW315448	Aphis spiraecola voucher A44 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
26	KY833260	Aphis spiraecola voucher BIOUG02439-F05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
27	KY842435	Aphis spiraecola voucher BIOUG02439-F06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
28	MH632732	Aphis spiraecola isolate AsPOMM cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
29	MG954458	Aphis spiraecola isolate Y11-1 cytochrome oxidase subunit 1 gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
30	KY833259	Aphis spiraecola voucher BIOUG02439-C07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
31	MH632733	Aphis spiraecola isolate AsCOUR cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
32	MH407717	Aphis spiraecola isolate AS34_F cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
33	KY841003	Aphis spiraecola voucher BIOUG02439-F07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
34	KY841618	Aphis spiraecola voucher BIOUG02439-C06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
35	KY841985	Aphis spiraecola voucher BIOUG02439-B04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
36	PQ164413	Aphis spiraecola isolate CITHAs7 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
37	MN319745	Aphis spiraecola voucher NIBGE APH-00642 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
38	PQ157581	Aphis spiraecola isolate CITHAs2 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
39	MG954457	Aphis spiraecola isolate Y10-3 cytochrome oxidase subunit 1 gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
40	MH407716	Aphis spiraecola isolate AH36_F cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
41	PQ164404	Aphis spiraecola isolate CITHAs6 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
42	KY844751	Aphis spiraecola voucher BIOUG02439-D03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
43	MN320037	Aphis spiraecola voucher NIBGE APH-00347 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
44	PQ157588	Aphis spiraecola isolate CITHAs3 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
45	MN320342	Aphis spiraecola voucher NIBGE APH-00453 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
46	MN319958	Aphis spiraecola voucher NIBGE APH-00664 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
47	EU701492	Aphis spiraecola voucher CNC#HEM039576 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
48	KY832517	Aphis spiraecola voucher BIOUG02439-G08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
49	MN319732	Aphis spiraecola voucher NIBGE APH-00439 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	655.0	0.00e+00	99.8%
50	KJ702456	Aphis spiraecola voucher ROAsAp3-14 cytochrome c oxidase subunit 1 (cox1) gene, partial cds; mitochondrial	657	99.8%	654.0	0.00e+00	99.8%
51	KR036921	Aphis spiraecola voucher BIOUG00906-G07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	654.0	0.00e+00	99.8%
52	MW315425	Aphis spiraecola voucher A21 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	651	98.9%	648.0	0.00e+00	99.8%
53	KR037466	Aphis spiraecola voucher CNC#HEM059491 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	648	98.5%	645.0	0.00e+00	99.8%
54	KY832954	Aphis spiraecola voucher BIOUG02527-E02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	647	98.3%	644.0	0.00e+00	99.8%
55	MN320039	Aphis spiraecola voucher NIBGE APH-00665 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	647	98.3%	644.0	0.00e+00	99.8%
56	KY831140	Aphis spiraecola voucher BIOUG02527-D08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	644	97.9%	641.0	0.00e+00	99.8%
57	KY586082	Aphis spiraecola isolate Gwalior_A8 cytochrome c oxidase subunit 1 (COI) gene, partial cds; mitochondrial	639	97.1%	636.0	0.00e+00	99.8%
58	KY586081	Aphis spiraecola isolate Gwalior_A7 cytochrome c oxidase subunit 1 (COI) gene, partial cds; mitochondrial	639	97.1%	636.0	0.00e+00	99.8%
59	KY586078	Aphis spiraecola isolate Gwalior_A3 cytochrome c oxidase subunit 1 (COI) gene, partial cds; mitochondrial	639	97.1%	636.0	0.00e+00	99.8%
60	KR030794	Aphis spiraecola voucher CNC#HEM071354 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	639	97.1%	636.0	0.00e+00	99.8%
61	KR032814	Aphis spiraecola voucher 10BBCHEM-0571 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	629	95.6%	626.0	0.00e+00	99.8%
62	MK547632	Aphis spiraecola isolate OAI726 cytochrome c oxidase subunit I (COI) gene, partial cds; mitochondrial	627	95.3%	624.0	0.00e+00	99.8%
63	KR031910	Aphis spiraecola voucher CNC#HEM070783 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	623	94.7%	620.0	0.00e+00	99.8%
64	KR042276	Aphis spiraecola voucher CNC#HEM070776 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	621	94.4%	618.0	0.00e+00	99.8%
65	MN320265	Aphis spiraecola voucher NIBGE APH-00199 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	614	93.3%	611.0	0.00e+00	99.8%
66	KJ467505	Aphis spiraecola cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	607	92.2%	604.0	0.00e+00	99.8%
67	MG027897	Aphis spiraecola isolate MVV121189.22 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	605	91.9%	602.0	0.00e+00	99.8%
68	MN320019	Aphis spiraecola voucher NIBGE APH-00217 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	604	91.8%	601.0	0.00e+00	99.8%
69	MN320330	Aphis spiraecola voucher NIBGE APH-00198 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	602	91.5%	599.0	0.00e+00	99.8%
70	MN320186	Aphis spiraecola voucher NIBGE APH-00200 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	602	91.5%	599.0	0.00e+00	99.8%
71	MN320198	Aphis spiraecola voucher NIBGE APH-00201 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	602	91.5%	599.0	0.00e+00	99.8%
72	MN319875	Aphis spiraecola voucher NIBGE APH-00265 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	602	91.5%	599.0	0.00e+00	99.8%
73	MN319786	Aphis spiraecola voucher NIBGE APH-00202 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	602	91.5%	599.0	0.00e+00	99.8%
74	MW412105	Aphis fabae isolate Ap12 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial.	598	90.9%	595.0	0.00e+00	99.8%
75	MW412103	Aphis spiraecola isolate Ap1 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial.	598	90.9%	595.0	0.00e+00	99.8%
76	MW412106	Aphis pomi isolate Ap13 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial.	595	90.4%	592.0	0.00e+00	99.8%
77	KR577026	Aphis sp. BOLD:AAA4183 voucher BIOUG07811-F12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	592	90.0%	589.0	0.00e+00	99.8%
78	KR566608	Aphis sp. BOLD:AAA4183 voucher BIOUG16167-B02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	591	89.8%	588.0	0.00e+00	99.8%
79	KR578850	Aphis spiraecola voucher BIOUG07158-E05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	589	89.5%	586.0	0.00e+00	99.8%
80	MG169271	Aphis spiraecola voucher BIOUG25754-F07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	588	89.4%	585.0	0.00e+00	99.8%
81	KR583489	Aphis spiraecola voucher BIOUG08805-B05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	583	88.6%	580.0	0.00e+00	99.8%
82	KR565197	Aphididae sp. BOLD:AAA4183 voucher BIOUG09129-C01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	582	88.4%	579.0	0.00e+00	99.8%
83	KR573224	Aphis spiraecola voucher BIOUG07898-D06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	579	88.0%	576.0	0.00e+00	99.8%
84	KR561329	Aphis sp. BOLD:AAA4183 voucher BIOUG07800-G12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	577	87.7%	574.0	0.00e+00	99.8%
85	KR037785	Aphis spiraecola voucher CHAN-2006-0202 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	576	87.5%	573.0	0.00e+00	99.8%
86	KR574458	Aphis spiraecola voucher BIOUG08805-C11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	572	86.9%	569.0	0.00e+00	99.8%
87	JN214361	Aphis spiraecola isolate Mc1 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	570	86.6%	567.0	0.00e+00	99.8%
88	MG166603	Aphis spiraecola voucher BIOUG27402-G02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	567	86.2%	564.0	0.00e+00	99.8%
89	OM611898	Aphis spiraecola voucher BIOUG13513-D03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	567	86.2%	564.0	0.00e+00	99.8%
90	OM576727	Aphis spiraecola voucher BIOUG22992-G02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	567	86.2%	564.0	0.00e+00	99.8%
91	OM546907	Aphis spiraecola voucher BIOUG12772-C12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	567	86.2%	564.0	0.00e+00	99.8%
92	KR560659	Aphididae sp. BOLD:AAA4183 voucher BIOUG08415-G07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	567	86.2%	564.0	0.00e+00	99.8%
93	MG168891	Aphis spiraecola voucher BIOUG23313-H04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	564	85.7%	561.0	0.00e+00	99.8%
94	MG167194	Aphis spiraecola voucher BIOUG25754-G07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	561	85.3%	558.0	0.00e+00	99.8%
95	MG169427	Aphis spiraecola voucher BIOUG22100-D03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	561	85.3%	558.0	0.00e+00	99.8%
96	MH821422	Aphis spiraecola voucher HL712 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
97	MH821522	Aphis spiraecola voucher HLZ208 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
98	MH821372	Aphis spiraecola voucher HL180 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
99	MH821406	Aphis spiraecola voucher HL479 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
100	MH821474	Aphis spiraecola voucher HLshujia617 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
101	MH821371	Aphis spiraecola voucher HL177 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
102	MH821535	Aphis spiraecola voucher HLZ82 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
103	MH821484	Aphis spiraecola voucher HLshujia712 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
104	MH821477	Aphis spiraecola voucher HLshujia65 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
105	MH821461	Aphis spiraecola voucher HLshujia485 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
106	MH821455	Aphis spiraecola voucher HLshujia408 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
107	MH821436	Aphis spiraecola voucher HLshujia275 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
108	MH821504	Aphis spiraecola voucher HLshujia86 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
109	MH821390	Aphis spiraecola voucher HL332 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
110	MH821382	Aphis spiraecola voucher HL291 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
111	MH821448	Aphis spiraecola voucher HLshujia372 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
112	MH821414	Aphis spiraecola voucher HL603 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
113	MH821426	Aphis spiraecola voucher HLshujia135 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
114	MH821439	Aphis spiraecola voucher HLshujia319 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
115	MH821407	Aphis spiraecola voucher HL500 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
116	MH821413	Aphis spiraecola voucher HL602 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
117	MH821395	Aphis spiraecola voucher HL343 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
118	MH821479	Aphis spiraecola voucher HLshujia658 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
119	MH821445	Aphis spiraecola voucher HLshujia356 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
120	MH821389	Aphis spiraecola voucher HL331 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
121	MH821376	Aphis spiraecola voucher HL201 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
122	MH821421	Aphis spiraecola voucher HL663 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
123	MH821488	Aphis spiraecola voucher HLshujia724 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
124	MH821527	Aphis spiraecola voucher HLZ288 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
125	MH821499	Aphis spiraecola voucher HLshujia801 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
126	MH821536	Aphis spiraecola voucher HLZ99 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
127	MH821531	Aphis spiraecola voucher HLZ379 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
128	MH821465	Aphis spiraecola voucher HLshujia503 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
129	MH821411	Aphis spiraecola voucher HL569 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
130	MH821435	Aphis spiraecola voucher HLshujia273 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
131	MH821481	Aphis spiraecola voucher HLshujia682 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
132	MH821403	Aphis spiraecola voucher HL453 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
133	MH821399	Aphis spiraecola voucher HL361 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
134	MH821443	Aphis spiraecola voucher HLshujia354 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
135	MH821373	Aphis spiraecola voucher HL183 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
136	MH821532	Aphis spiraecola voucher HLZ388 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
137	MH821510	Aphis spiraecola voucher HLY_47 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
138	MH821369	Aphis spiraecola voucher HL101 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
139	MH821374	Aphis spiraecola voucher HL195 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
140	MH821381	Aphis spiraecola voucher HL275 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
141	MH821520	Aphis spiraecola voucher HLZ201 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
142	MH821511	Aphis spiraecola voucher HLYam_60 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
143	MH821400	Aphis spiraecola voucher HL378 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
144	MH821500	Aphis spiraecola voucher HLshujia807 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
145	MH821396	Aphis spiraecola voucher HL344 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
146	MH821424	Aphis spiraecola voucher HLshujia131 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
147	MH821507	Aphis spiraecola voucher HLshujia891 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
148	MH821469	Aphis spiraecola voucher HLshujia577 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
149	MH821444	Aphis spiraecola voucher HLshujia355 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
150	MH821394	Aphis spiraecola voucher HL341 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
151	MH821388	Aphis spiraecola voucher HL330 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
152	MH821526	Aphis spiraecola voucher HLZ283 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
153	MH821529	Aphis spiraecola voucher HLZ378 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
154	MH821523	Aphis spiraecola voucher HLZ245 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
155	MH821398	Aphis spiraecola voucher HL347 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
156	MH821410	Aphis spiraecola voucher HL568 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
157	MH821446	Aphis spiraecola voucher HLshujia357 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
158	MH821434	Aphis spiraecola voucher HLshujia247 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
159	MH821423	Aphis spiraecola voucher HLshujia124 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
160	MH821521	Aphis spiraecola voucher HLZ207 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
161	MH821468	Aphis spiraecola voucher HLshujia534 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
162	MH821391	Aphis spiraecola voucher HL336 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
163	MH821377	Aphis spiraecola voucher HL212 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
164	MH821534	Aphis spiraecola voucher HLZ63 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
165	MH821458	Aphis spiraecola voucher HLshujia437 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
166	MH821420	Aphis spiraecola voucher HL652 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
167	MH821380	Aphis spiraecola voucher HL250 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
168	MH821378	Aphis spiraecola voucher HL232 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
169	MH821450	Aphis spiraecola voucher HLshujia387 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
170	MH821393	Aphis spiraecola voucher HL339 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
171	MH821387	Aphis spiraecola voucher HL318 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	557.0	0.00e+00	99.8%
172	KY835683	Aphis spiraecola voucher BIOUG15298-H03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	555	84.3%	552.0	0.00e+00	99.8%
173	MK547637	Aphis spiraecola isolate OAI735 cytochrome c oxidase subunit I (COI) gene, partial cds; mitochondrial	555	84.3%	552.0	0.00e+00	99.8%
174	OM550376	Aphis spiraecola voucher BIOUG22479-D07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	555	84.3%	552.0	0.00e+00	99.8%
175	OM545779	Aphis spiraecola voucher BIOUG22479-B11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	555	84.3%	552.0	0.00e+00	99.8%
176	OM551684	Aphis spiraecola voucher BIOUG12772-C10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	555	84.3%	552.0	0.00e+00	99.8%
177	KY836784	Aphis spiraecola voucher BIOUG17840-D05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	555	84.3%	552.0	0.00e+00	99.8%
178	KY845891	Aphis spiraecola voucher BIOUG15390-B02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	549	83.4%	546.0	0.00e+00	99.8%
179	MG167587	Aphis spiraecola voucher BIOUG24149-B03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	549	83.4%	546.0	0.00e+00	99.8%
180	OM551014	Aphis spiraecola voucher BIOUG22479-C07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	546	83.0%	543.0	0.00e+00	99.8%
181	KR583353	Aphis spiraecola voucher BIOUG07903-F11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	546	83.0%	543.0	0.00e+00	99.8%
182	MN319979	Aphis spiraecola voucher NIBGE APH-00633 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	653.0	0.00e+00	99.7%
183	MN320202	Aphis spiraecola voucher NIBGE APH-00557 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	653.0	0.00e+00	99.7%
184	HQ112181	Aphis spiraecola voucher ORP-2010-46 cytochrome oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	652.0	0.00e+00	99.7%
185	FJ998564	Aphis spiraecola voucher CNC#HEM039286 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	652.0	0.00e+00	99.7%
186	MH632730	Aphis spiraecola isolate AsCITR cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	658	100.0%	652.0	0.00e+00	99.7%
187	KP759543	Aphis spiraecola voucher As G1-269 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	652.0	0.00e+00	99.7%
188	MG954456	Aphis spiraecola isolate Y2-4 cytochrome oxidase subunit 1 gene, partial cds; mitochondrial	658	100.0%	652.0	0.00e+00	99.7%
189	KP759544	Aphis spiraecola voucher As Pa-248 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	652.0	0.00e+00	99.7%
190	AB506736	Aphis spiraecola mitochondrial COI gene for cytochrome oxidase subunit I, partial cds, isolate: Aspi2	658	100.0%	652.0	0.00e+00	99.7%
191	KY586083	Aphis spiraecola isolate Jalandhar_A2 cytochrome c oxidase subunit 1 (COI) gene, partial cds; mitochondrial	639	97.1%	633.0	0.00e+00	99.7%
192	KY586085	Aphis spiraecola isolate Modipuram_A43 cytochrome c oxidase subunit 1 (COI) gene, partial cds; mitochondrial	639	97.1%	633.0	0.00e+00	99.7%
193	KM115471	Aphis pomi voucher ZMIOZ LY073 cytochrome c oxidase subunit I (COI) gene, partial cds; mitochondrial	631	95.9%	625.0	0.00e+00	99.7%
194	KM115468	Aphis pomi voucher ZMIOZ LY074 cytochrome c oxidase subunit I (COI) gene, partial cds; mitochondrial	628	95.4%	622.0	0.00e+00	99.7%
195	MH407726	Aphis spiraecola isolate AS14_F cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	626	95.1%	620.0	0.00e+00	99.7%
196	KJ502194	Aphis spiraecola voucher 110728WH04 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	609	92.6%	603.0	0.00e+00	99.7%
197	MN320062	Aphis spiraecola voucher NIBGE APH-00216 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	604	91.8%	599.0	0.00e+00	99.7%
198	MN320160	Aphis spiraecola voucher NIBGE APH-00695 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	590	89.7%	584.0	0.00e+00	99.7%
199	MH821401	Aphis spiraecola voucher HL416 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	554.0	0.00e+00	99.6%
200	MH821493	Aphis spiraecola voucher HLshujia748 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	554.0	0.00e+00	99.6%
201	MH821467	Aphis spiraecola voucher HLshujia518 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	554.0	0.00e+00	99.6%
202	MH821466	Aphis spiraecola voucher HLshujia51 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	554.0	0.00e+00	99.6%
203	MH821503	Aphis spiraecola voucher HLshujia846 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	554.0	0.00e+00	99.6%
204	MH821402	Aphis spiraecola voucher HL445 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	554.0	0.00e+00	99.6%
205	MH821370	Aphis spiraecola voucher HL139 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	554.0	0.00e+00	99.6%
206	MH821489	Aphis spiraecola voucher HLshujia726 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	554.0	0.00e+00	99.6%
207	MH821427	Aphis spiraecola voucher HLshujia139 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	554.0	0.00e+00	99.6%
208	KY838627	Aphis spiraecola voucher BIOUG17840-F05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	555	84.3%	550.0	0.00e+00	99.6%
209	MH821447	Aphis spiraecola voucher HLshujia361 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	551	83.7%	545.0	0.00e+00	99.6%
210	JQ916116	Aphis gossypii voucher ZMIOZ 15832 cytochrome oxidase subunit 1 gene, partial cds; mitochondrial	658	100.0%	649.0	0.00e+00	99.5%
211	AB506737	Aphis spiraecola mitochondrial COI gene for cytochrome oxidase subunit I, partial cds, isolate: Aspi3	658	100.0%	649.0	0.00e+00	99.5%
212	FJ998576	Aphis spiraecola voucher CNC#HEM054297 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	649.0	0.00e+00	99.5%
213	KY838533	Aphis spiraecola voucher BIOUG02533-F01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	640	97.3%	631.0	0.00e+00	99.5%
214	KJ502195	Aphis spiraecola voucher 110718WH39 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	630	95.7%	621.0	0.00e+00	99.5%
215	KR573495	Aphis spiraecola voucher BIOUG08421-H11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	591	89.8%	582.0	0.00e+00	99.5%
216	KR576913	Aphis spiraecola voucher BIOUG07279-G02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	589	89.5%	580.0	0.00e+00	99.5%
217	KR573647	Aphis spiraecola voucher BIOUG16066-G04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	573	87.1%	564.0	0.00e+00	99.5%
218	MH821415	Aphis spiraecola voucher HL604 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	551.0	0.00e+00	99.5%
219	MH821525	Aphis spiraecola voucher HLZ259 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	551.0	0.00e+00	99.5%
220	MH821485	Aphis spiraecola voucher HLshujia713 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	551.0	0.00e+00	99.5%
221	MH821428	Aphis spiraecola voucher HLshujia140 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	551.0	0.00e+00	99.5%
222	KY834103	Aphis spiraecola voucher BIOUG15298-D04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	555	84.3%	548.0	0.00e+00	99.5%
223	MN320152	Aphis spiraecola voucher NIBGE APH-00586 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	649.0	0.00e+00	99.4%
224	JX844401	Aphis pomi voucher ZMIOZ 23256 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
225	FJ998581	Aphis spiraecola voucher CNC#HEM056615 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
226	MG954459	Aphis spiraecola isolate Y11-2 cytochrome oxidase subunit 1 gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
227	MT445569	Aphis spiraecola isolate Trdosovce (SK) cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
228	JX844372	Aphis spiraecola voucher ZMIOZ 14330 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
229	MT445571	Aphis spiraecola isolate Sittingbourne (UK) cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
230	MT445572	Aphis spiraecola isolate Bile Podoli (CZ) cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
231	MT445570	Aphis spiraecola isolate West Malling (UK) cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
232	MT445573	Aphis spiraecola isolate Mihalyi (HU) cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
233	MT445567	Aphis spiraecola isolate Llugaxhi orchard 2 (KOS) cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
234	MT445577	Aphis spiraecola isolate East Malling (UK) cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
235	MZ662895	Aphis spiraecola isolate YamAphid-5A-YWA-2020 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
236	MT445575	Aphis spiraecola isolate Holovousy orchard 1 (CZ) cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
237	MT445576	Aphis spiraecola isolate Holovousy orchard 2 (CZ) cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
238	MZ662877	Aphis spiraecola isolate YamAphid-2L-YWA-2017 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
239	MZ662882	Aphis spiraecola isolate YamAphid-2B-YWA-2018 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
240	MZ662878	Aphis spiraecola isolate YamAphid-3A-YWA-2017 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
241	MZ662876	Aphis spiraecola isolate YamAphid-1L-YWA-2017 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
242	MT445568	Aphis spiraecola isolate Llugaxhi orchard 1 (KOS) cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	646.0	0.00e+00	99.4%
243	KM115473	Aphis pomi voucher ZMIOZ LY076 cytochrome c oxidase subunit I (COI) gene, partial cds; mitochondrial	646	98.2%	634.0	0.00e+00	99.4%
244	MW412104	Aphis fabae isolate Ap11 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial.	598	90.9%	586.0	0.00e+00	99.3%
245	MW412111	Aphis pomi isolate Ap19 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial.	598	90.9%	586.0	0.00e+00	99.3%
246	MW412112	Aphis fabae isolate Ap3 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial.	598	90.9%	586.0	0.00e+00	99.3%
247	MW412113	Aphis fabae isolate Ap4 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial.	574	87.2%	562.0	0.00e+00	99.3%
248	JN214362	Aphis spiraecola isolate Mc2 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	570	86.6%	558.0	0.00e+00	99.3%
249	OM611433	Aphis spiraecola voucher BIOUG13513-E06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	567	86.2%	555.0	0.00e+00	99.3%
250	MH821379	Aphis spiraecola voucher HL241 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	548.0	0.00e+00	99.3%
251	MH821383	Aphis spiraecola voucher HL314 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	548.0	0.00e+00	99.3%
252	MH821502	Aphis spiraecola voucher HLshujia827 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	548.0	0.00e+00	99.3%
253	MH821482	Aphis spiraecola voucher HLshujia692 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	548.0	0.00e+00	99.3%
254	MH821494	Aphis spiraecola voucher HLshujia775 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	548.0	0.00e+00	99.3%
255	MH821501	Aphis spiraecola voucher HLshujia808 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	548.0	0.00e+00	99.3%
256	MH821496	Aphis spiraecola voucher HLshujia792 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	548.0	0.00e+00	99.3%
257	MH821478	Aphis spiraecola voucher HLshujia651 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	548.0	0.00e+00	99.3%
258	MH821432	Aphis spiraecola voucher HLshujia194 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	548.0	0.00e+00	99.3%
259	MH821433	Aphis spiraecola voucher HLshujia197 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	548.0	0.00e+00	99.3%
260	MH821498	Aphis spiraecola voucher HLshujia800 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	548.0	0.00e+00	99.3%
261	MH821384	Aphis spiraecola voucher HL315 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	548.0	0.00e+00	99.3%
262	MH821491	Aphis spiraecola voucher HLshujia736 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	548.0	0.00e+00	99.3%
263	PP917990	Aphis spiraecola voucher ZGMB HJAs cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	643.0	0.00e+00	99.2%
264	MH714463	Aphis spiraecola voucher AP15 cytochrome oxidase subunit 1 (CO1) gene, partial cds; mitochondrial	642	97.6%	627.0	0.00e+00	99.2%
265	KY586079	Aphis spiraecola isolate Gwalior_A5 cytochrome c oxidase subunit 1 (COI) gene, partial cds; mitochondrial	639	97.1%	624.0	0.00e+00	99.2%
266	ON461370	Aphis spiraecola voucher 0821 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	619	94.1%	604.0	0.00e+00	99.2%
267	MK184484	Aphis pomi voucher AP0V11 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	600	91.2%	585.0	0.00e+00	99.2%
268	MK281581	Aphis pomi cytochrome oxidase subunit 1 (CO1) gene, partial cds; mitochondrial	600	91.2%	585.0	0.00e+00	99.2%
269	MZ662881	Aphis spiraecola isolate YamAphid-2A-YWA-2018 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	640.0	0.00e+00	99.1%
270	MH632731	Aphis spiraecola isolate AsHIBI cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	658	100.0%	640.0	0.00e+00	99.1%
271	KR856185	Aphis spiraecola cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	658	100.0%	640.0	0.00e+00	99.1%
272	KM115472	Aphis pomi voucher ZMIOZ LY075 cytochrome c oxidase subunit I (COI) gene, partial cds; mitochondrial	647	98.3%	629.0	0.00e+00	99.1%
273	ON368711	Aphis spiraecola voucher 200317amH43 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	582	88.4%	567.0	0.00e+00	99.1%
274	MH821417	Aphis spiraecola voucher HL631 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
275	MH821513	Aphis spiraecola voucher HLZ122 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
276	MH821409	Aphis spiraecola voucher HL563 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
277	MH821524	Aphis spiraecola voucher HLZ258 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
278	MH821533	Aphis spiraecola voucher HLZ47 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
279	MH821412	Aphis spiraecola voucher HL584 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
280	MH821487	Aphis spiraecola voucher HLshujia720 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
281	MH821515	Aphis spiraecola voucher HLZ171 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
282	MH821437	Aphis spiraecola voucher HLshujia283 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
283	MH821517	Aphis spiraecola voucher HLZ177 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
284	MH821480	Aphis spiraecola voucher HLshujia665 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
285	MH821418	Aphis spiraecola voucher HL639 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
286	MH821514	Aphis spiraecola voucher HLZ132 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
287	MH821425	Aphis spiraecola voucher HLshujia132 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
288	MH821416	Aphis spiraecola voucher HL614 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
289	MH821492	Aphis spiraecola voucher HLshujia742 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
290	MH821512	Aphis spiraecola voucher HLZ120 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	545.0	0.00e+00	99.1%
291	KY586084	Aphis spiraecola isolate Jalandhar A3 cytochrome c oxidase subunit 1 (COI) gene, partial cds; mitochondrial	639	97.1%	618.0	0.00e+00	98.9%
292	MH821528	Aphis spiraecola voucher HLZ377 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	560	85.1%	542.0	0.00e+00	98.9%
293	EU701320	Aphis sp. BOLD:AAA4183 voucher CNC#HEM113514 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	616.0	0.00e+00	97.9%
294	MK241551	Aphis spiraecola voucher AP0E12 cytochrome oxidase subunit 1 (CO1) gene, partial cds; mitochondrial	600	91.2%	555.0	0.00e+00	97.5%
295	KM115512	Aphis spiraecola voucher ZMIOZ ZZM008 cytochrome c oxidase subunit I (COI) gene, partial cds; mitochondrial	649	98.6%	598.0	0.00e+00	97.4%
296	KP189463	Aphis spiraecola voucher ORP SP-LK146 cytochrome oxidase subunit 1 (COX1) gene, partial cds; mitochondrial	658	100.0%	604.0	0.00e+00	97.3%
297	DQ499028	Aphis spiraecola cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	658	100.0%	600.0	0.00e+00	97.1%
298	AB506738	Aphis spiraecola mitochondrial COI gene for cytochrome oxidase subunit I, partial cds, isolate: Aspi4	658	100.0%	598.0	0.00e+00	97.0%
299	KY586080	Aphis spiraecola isolate Gwalior_A6 cytochrome c oxidase subunit 1 (COI) gene, partial cds; mitochondrial	639	97.1%	579.0	0.00e+00	96.9%
300	JQ916095	Aphis spiraecola voucher ZMIOZ 13737 cytochrome oxidase subunit 1 gene, partial cds; mitochondrial	657	99.8%	564.0	0.00e+00	95.3%
301	KR034120	Aphis maculatae voucher CHU06-APH-168 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	645	98.0%	555.0	0.00e+00	95.3%
302	KR038391	Aphis maculatae voucher CHU06-APH-086 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	645	98.0%	555.0	0.00e+00	95.3%
303	KR033268	Aphis maculatae voucher CHU06-APH-147 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	645	98.0%	555.0	0.00e+00	95.3%
304	MT445574	Aphis pomi isolate Mihalyi (HU) cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	622	94.5%	535.0	0.00e+00	95.3%
305	EU701544	Braggia urovaneta voucher CNC#HEM054170 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	562.0	0.00e+00	95.1%
306	EU701446	Aphis maculatae voucher CNC#HEM006908 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	562.0	0.00e+00	95.1%
307	KR032354	Aphis maculatae voucher CNC#HEM056835 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	561.0	0.00e+00	95.1%
308	EU701476	Aphis pomi voucher CNC#HEM039277 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	561.0	0.00e+00	95.1%
309	EU701478	Aphis pomi voucher CNC#HEM033921 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	561.0	0.00e+00	95.1%
310	KR039601	Aphis maculatae voucher CNC#HEM057465 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	559.0	0.00e+00	95.0%
311	KF638995	Aphis pomi voucher INRA CBGP ACOE1257/chasses1257-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	558.0	0.00e+00	95.0%
312	KR042798	Aphis pomi voucher CNC#HEM054263 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	558.0	0.00e+00	95.0%
313	GQ904107	Aphis neospiraeae isolate 030523SH25 cytochrome c oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	558.0	0.00e+00	95.0%
314	KR033472	Aphis maculatae voucher CNC#HEM059939 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	655	99.5%	556.0	0.00e+00	95.0%
315	HQ900843	Aphis pomi isolate FUM 6 cytochrome oxidase subunit 1 gene, partial cds; mitochondrial	638	97.0%	542.0	0.00e+00	95.0%
316	MT302350	Aphis pomi voucher 3_n1 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	635	96.5%	539.0	0.00e+00	95.0%
317	PP627400	Aphis pomi isolate CITHAp1 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	635	96.5%	539.0	0.00e+00	95.0%
318	MT302349	Aphis pomi voucher 2_g1 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	635	96.5%	539.0	0.00e+00	95.0%
319	HM416737	Aphis pomi voucher CNC#HEM064282 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	634	96.4%	538.0	0.00e+00	95.0%
320	FJ998516	Aphis pomi voucher CNC#HEM032174 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	557.0	0.00e+00	94.8%
321	KF638749	Aphis brotericola voucher INRA CBGP ACOE1449/chasses1449-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	556.0	0.00e+00	94.8%
322	OR091476	Aphis celastrii isolate S09 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	556.0	0.00e+00	94.8%
323	MT302351	Aphis pomi voucher 1_k1 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	635	96.5%	536.0	0.00e+00	94.8%
324	EU701546	Braggia urovaneta voucher CNC#HEM113654 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	553.0	0.00e+00	94.7%
325	EU701545	Braggia urovaneta voucher CNC#HEM054158 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	553.0	0.00e+00	94.7%
326	JQ916118	Aphis kurosawai voucher ZMIOZ 15982 cytochrome oxidase subunit 1 gene, partial cds; mitochondrial	658	100.0%	553.0	0.00e+00	94.7%
327	GQ904106	Aphis kurosawai isolate 050603HJ16 cytochrome c oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	550.0	0.00e+00	94.5%
328	KF639103	Aphis verbasci voucher INRA CBGP ACOE2026/chasses2026-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	547.0	0.00e+00	94.4%
329	JQ916098	Aphis anuraphoides voucher ZMIOZ 13874 cytochrome oxidase subunit 1 gene, partial cds; mitochondrial	651	98.9%	540.0	0.00e+00	94.3%
330	KR041033	Aphis astragalina voucher CNC#HEM039981 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	544.0	0.00e+00	94.2%
331	HM432515	Aphis sp. RFBAE387-09 voucher CNC#HEM063328 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	544.0	0.00e+00	94.2%
332	EU701537	Brachyunguis tetrapteralis voucher CNC#HEM054151 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	544.0	0.00e+00	94.2%
333	EU701540	Braggia eriogoni voucher CNC#HEM026463 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	544.0	0.00e+00	94.2%
334	KF639104	Aphis verbasci voucher INRA CBGP ACOE432/chasses432-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	544.0	0.00e+00	94.2%
335	KF639099	Aphis vallei voucher INRA CBGP ACOE2444/chasses2444-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	544.0	0.00e+00	94.2%
336	KF639098	Aphis vallei voucher INRA CBGP ACOE1475/chasses1475-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	544.0	0.00e+00	94.2%
337	KF638912	Aphis galiiscabri voucher INRA CBGP ACOE1612/chasses1612-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	544.0	0.00e+00	94.2%
338	KF639380	Ephedraphis ephedrae voucher INRA CBGP ACOE575/chasses575-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	544.0	0.00e+00	94.2%
339	KR571863	Aphis sp. BOLD-2016 voucher BIOUG04209-A04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	544.0	0.00e+00	94.2%
340	GQ377866	Braggia eriogoni voucher CNC#HEM061765 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	544.0	0.00e+00	94.2%
341	GU668704	Aphis astragalina voucher CNC#HEM063715 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	544.0	0.00e+00	94.2%
342	MN671896	Aphis sp. BOLD:ACF2671 voucher CHARS00039-C07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	654	99.4%	540.0	0.00e+00	94.2%
343	MF834058	Aphidinae sp. BIOUG14779-E10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	651	98.9%	537.0	0.00e+00	94.2%
344	EU701541	Braggia eriogoni voucher CNC#HEM033259 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	541.0	0.00e+00	94.1%
345	KR032325	Aphis helianthi voucher CNC#HEM028897 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	541.0	0.00e+00	94.1%
346	KF638797	Aphis cytisorum voucher INRA CBGP ACOE1505/chasses1505-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	541.0	0.00e+00	94.1%
347	HQ970842	Aphis helianthi voucher CNC#HEM070009 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	541.0	0.00e+00	94.1%
348	KY323025	Aphis fabae voucher AfKr5 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	541.0	0.00e+00	94.1%
349	KF639106	Aphis verbasci voucher INRA CBGP ACOE461/chasses461-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	541.0	0.00e+00	94.1%
350	KC502060	Aphididae sp. BOLD:ABZ4520 voucher CCDB-08110-D02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	541.0	0.00e+00	94.1%
351	JF883731	Aphis helianthi voucher CNC#HEM070712 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	541.0	0.00e+00	94.1%
352	KF638799	Aphis cytisorum voucher INRA CBGP ACOE1959/chasses1959-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	541.0	0.00e+00	94.1%
353	EU701538	Braggia eriogoni voucher CNC#HEM055868 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	541.0	0.00e+00	94.1%
354	JQ916094	Aphis lhasaensis voucher ZMIOZ 13542 cytochrome oxidase subunit 1 gene, partial cds; mitochondrial	658	100.0%	541.0	0.00e+00	94.1%
355	KF638913	Aphis galiiscabri voucher INRA CBGP ACOE2012/chasses2012-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	541.0	0.00e+00	94.1%
356	EU701539	Braggia eriogoni voucher CNC#HEM028589 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	541.0	0.00e+00	94.1%
357	KF638751	Aphis caroliboerneri voucher INRA CBGP ACOE2478/chasses2478-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	541.0	0.00e+00	94.1%
358	MF831318	Aphis rumicis voucher BIOUG27467-C08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	540.0	0.00e+00	94.1%
359	KF638755	Aphis clematidis voucher INRA CBGP ACOE2482/chasses2482-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	540.0	0.00e+00	94.1%
360	KR045155	Aphis astragalina voucher BIOUG02022-F04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	539.0	0.00e+00	93.9%
361	KY845876	Aphis craccivora voucher BIOUG02533-H05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
362	GQ904116	Aphis oenotherae isolate 030625SH67 cytochrome c oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
363	KF638779	Aphis craccivora voucher INRA CBGP ACOE1410/chasses1410-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
364	MZ444691	Aphis rogeri voucher NZMC_aphid 46792 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
365	JQ916144	Aphis lhasaensis voucher ZMIOZ 17309 cytochrome oxidase subunit 1 gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
366	KR031480	Aphis helianthi voucher CNC#HEM114036 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
367	GQ377860	Braggia agathona voucher CNC#HEM061783 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
368	FJ429946	Braggia eriogoni voucher CNC#HEM058074 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
369	HQ970637	Aphis astragalina voucher CNC#HEM070099 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
370	PP952032	Aphis craccivora strain AcLc_AM cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
371	MN320340	Aphis craccivora voucher NIBGE APH-00296 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
372	MN319929	Aphis craccivora voucher NIBGE APH-00065 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
373	AB506711	Aphis craccivora mitochondrial COI gene for cytochrome oxidase subunit I, partial cds, isolate: Acra1	658	100.0%	538.0	0.00e+00	93.9%
374	KR569478	Aphis pentstemonicola voucher BIOUG03700-D11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
375	KF638800	Aphis cytisorum voucher INRA CBGP ACOE1993/chasses1993-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
376	MN319944	Aphis craccivora voucher NIBGE APH-00380 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
377	KY830692	Aphis craccivora voucher BIOUG02439-H08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
378	MZ444694	Aphis rogeri voucher NZMC_aphid 49524 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
379	HQ971231	Aphis viburniphila voucher CNC#HEM064419 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
380	KY322947	Aphis craccivora voucher AfLa3 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
381	KF639085	Aphis ulicis voucher INRA CBGP ACOE1305/chasses1305-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
382	MN319823	Aphis craccivora voucher NIBGE APH-00146 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
383	KY835106	Aphis craccivora voucher BIOUG02439-H10 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
384	KR565376	Aphis pentstemonicola voucher BIOUG05351-C05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	538.0	0.00e+00	93.9%
385	MK547618	Aphis craccivora isolate OAI716 cytochrome c oxidase subunit I (COI) gene, partial cds; mitochondrial	657	99.8%	537.0	0.00e+00	93.9%
386	MN319854	Brachyunguis harmalae voucher NIBGE APH-00311 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	537.0	0.00e+00	93.9%
387	EU701448	Aphis manitobensis voucher CNC#HEM007694 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	537.0	0.00e+00	93.9%
388	HM405597	Aphis schneideri voucher CGAEC-125 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	537.0	0.00e+00	93.9%
389	MT449693	Aphis rumicis isolate Polygonaceae-Dokdo cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	657	99.8%	537.0	0.00e+00	93.9%
390	OR098298	Aphis sp. isolate B2 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	657	99.8%	537.0	0.00e+00	93.9%
391	MN320122	Brachyunguis harmalae voucher NIBGE APH-00153 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	537.0	0.00e+00	93.9%
392	HQ970728	Aphis oenotherae voucher CNC#HEM007153 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	537.0	0.00e+00	93.9%
393	JN644996	Mollitrichosiphum tenuicorpus voucher ZMIOZ 22159 cytochrome c oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	537.0	0.00e+00	93.9%
394	MN319899	Brachyunguis harmalae voucher NIBGE APH-00152 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	537.0	0.00e+00	93.9%
395	KR042803	Aphis varians voucher CNC#HEM061460 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	537.0	0.00e+00	93.9%
396	EU701295	Aphis acaenovinae voucher CNC#HEM053814 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	657	99.8%	537.0	0.00e+00	93.9%
397	AB506734	Aphis rumicis mitochondrial COI gene for cytochrome oxidase subunit I, partial cds, isolate: Arum	657	99.8%	537.0	0.00e+00	93.9%
398	JQ916121	Aphis rumicis voucher ZMIOZ 16193 cytochrome oxidase subunit 1 gene, partial cds; mitochondrial	657	99.8%	537.0	0.00e+00	93.9%
399	JQ916145	Aphis rumicis voucher ZMIOZ 17310 cytochrome oxidase subunit 1 gene, partial cds; mitochondrial	657	99.8%	537.0	0.00e+00	93.9%
400	EU701481	Aphis rubifolii voucher CNC#HEM049296 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	536.0	0.00e+00	93.8%
401	EU701480	Aphis rubifolii voucher CNC#HEM049300 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	536.0	0.00e+00	93.8%
402	KR562124	Aphis glycines voucher BIOUG01607-D01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	536.0	0.00e+00	93.8%
403	JQ916112	Toxoptera aurantii voucher ZMIOZ 15645 cytochrome oxidase subunit 1 gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
404	KR568414	Braggia sp. BOLD-2016 voucher BIOUG03541-E11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
405	KY846952	Aphis craccivora voucher BIOUG02533-B08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
406	MN320267	Aphis craccivora voucher NIBGE APH-00133 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
407	MN320178	Aphis craccivora voucher NIBGE APH-00352 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
408	MW491813	Aphis craccivora isolate NM60 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
409	MN320244	Aphis craccivora voucher NIBGE APH-00419 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
410	GU324658	Aphis craccivora voucher ZMCAS 19963 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
411	MW491811	Aphis craccivora isolate NM16 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
412	KX371821	Aphis craccivora voucher ROApcracc2-16 cytochrome c oxidase subunit 1 (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
413	MH407713	Aphis craccivora isolate AC363_F cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
414	KT889380	Aphis glycines isolate Zhengzhou mitochondrion, partial genome	658	100.0%	535.0	0.00e+00	93.8%
415	MN320204	Aphis craccivora voucher NIBGE APH-00103 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
416	MW491791	Aphis craccivora isolate NM37 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
417	KR031842	Aphis glycines voucher HUM-2006-1300 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
418	KY834859	Aphis craccivora voucher BIOUG02439-H04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
419	KY836830	Aphis craccivora voucher BIOUG02439-E02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
420	MW491804	Aphis craccivora isolate NM33 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
421	MW491797	Aphis craccivora isolate NM53 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
422	MN319870	Aphis craccivora voucher NIBGE APH-00318 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
423	MN320190	Aphis craccivora voucher NIBGE APH-00551 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
424	KR037787	Aphis viburniphila voucher CNC#HEM071306 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
425	MN320347	Aphis craccivora voucher NIBGE APH-00550 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
426	NC_031387	Aphis craccivora isolate Old_world mitochondrion, complete genome	658	100.0%	535.0	0.00e+00	93.8%
427	MZ662890	Aphis craccivora isolate Aphid-10A-YWA-2019 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
428	AB506723	Aphis glycines mitochondrial COI gene for cytochrome oxidase subunit I, partial cds, isolate: Agly1	658	100.0%	535.0	0.00e+00	93.8%
429	KY845926	Aphis craccivora voucher BIOUG02527-A09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
430	MZ444686	Aphis rogeri voucher NZMC_aphid 46781 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
431	MN319773	Aphis craccivora voucher NIBGE APH-00330 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
432	KY834492	Aphis craccivora voucher BIOUG02533-B09 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
433	MN320110	Aphis craccivora voucher NIBGE APH-00573 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
434	MN319780	Aphis craccivora voucher NIBGE APH-00298 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
435	KR085004	Aphis craccivora voucher Ac5 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
436	MN320078	Aphis craccivora voucher CCDB-23161-H08 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
437	MW491798	Aphis craccivora isolate NM52 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
438	MW491790	Aphis craccivora isolate NM51 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
439	EU701427	Aphis helianthi voucher CNC#HEM112781 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
440	MW491801	Aphis craccivora isolate NM29 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
441	KY838020	Aphis craccivora voucher BIOUG02134-F03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
442	KY837367	Aphis craccivora voucher BIOUG02439-C05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
443	AB506725	Aphis glycines mitochondrial COI gene for cytochrome oxidase subunit I, partial cds, isolate: Agly3	658	100.0%	535.0	0.00e+00	93.8%
444	KY842424	Aphis craccivora voucher BIOUG02439-G04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
445	MN320235	Aphis craccivora voucher NIBGE APH-00293 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
446	GU324628	Aphis rumicis voucher ZMCAS 16309 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
447	KR017752	Aphis craccivora voucher DOGR 2 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
448	KY833152	Aphis craccivora voucher BIOUG02527-A07 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
449	KR038668	Aphis asclepiadis voucher CNC#HEM055765 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
450	MN319842	Aphis craccivora voucher NIBGE APH-00424 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
451	MW491808	Aphis craccivora isolate NM47 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
452	GQ377862	Braggia agathona voucher CNC#HEM061776 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
453	OR888763	Aphis craccivora isolate Ros3Tsr1 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
454	MN319955	Aphis craccivora voucher NIBGE APH-00101 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
455	MN319990	Aphis craccivora voucher NIBGE APH-00446 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
456	KY831028	Aphis craccivora voucher BIOUG02527-D02 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
457	MN319894	Aphis craccivora voucher NIBGE APH-00518 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
458	MN319749	Aphis craccivora voucher CCDB-23161-H04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
459	KF639084	Aphis ulicis voucher INRA CBGP ACOE1022/chasses1022-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
460	MN320307	Aphis craccivora voucher NIBGE APH-00310 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
461	KR043749	Aphis viburniphila voucher CNC#HEM007557 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
462	MN320318	Aphis craccivora voucher NIBGE APH-00132 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
463	MW491795	Aphis craccivora isolate NM48 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
464	EU701309	Aphis craccivora voucher CNC#HEM055010 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
465	GQ377859	Braggia agathona voucher CNC#HEM061784 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
466	KC840675	Aphis glycines mitochondrion, partial genome	658	100.0%	535.0	0.00e+00	93.8%
467	KR084996	Aphis craccivora voucher Ac9 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
468	KR084998	Aphis craccivora voucher Ac7 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
469	MN320070	Aphis craccivora voucher NIBGE APH-00412 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
470	MT095075	Aphis craccivora mitochondrion, complete genome	658	100.0%	535.0	0.00e+00	93.8%
471	MN320291	Aphis craccivora voucher CCDB-23161-H06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
472	NC_045236	Aphis glycines voucher FSCA:2018.5677 mitochondrion, complete genome	658	100.0%	535.0	0.00e+00	93.8%
473	MN319804	Aphis craccivora voucher NIBGE APH-00423 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
474	MN320343	Aphis craccivora voucher NIBGE APH-00320 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
475	KY831241	Aphis craccivora voucher BIOUG02439-E01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
476	MN319982	Aphis craccivora voucher NIBGE APH-00131 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
477	MN320346	Aphis craccivora voucher NIBGE APH-00517 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
478	MW491809	Aphis craccivora isolate NM42 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
479	KF638801	Aphis cytisorum voucher INRA CBGP ACOE2275/chasses2275-ACDA cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
480	KY844137	Aphis craccivora voucher BIOUG02439-G11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
481	GQ904082	Aphis craccivora isolate 031026HJ1 cytochrome c oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
482	MZ444692	Aphis rogeri voucher NZMC_aphid 47585 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
483	JQ920913	Aphis glycines voucher ZMIOZ 1994 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
484	MN320253	Aphis craccivora voucher NIBGE APH-00441 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
485	MN319756	Aphis craccivora voucher CCDB-23161-H05 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
486	MW491810	Aphis craccivora isolate NM40 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
487	MN320092	Aphis craccivora voucher NIBGE APH-00319 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
488	MN319890	Aphis craccivora voucher NIBGE APH-00345 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
489	MW491792	Aphis craccivora isolate NM43 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
490	KY846980	Aphis craccivora voucher BIOUG02439-F04 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
491	MW491789	Aphis craccivora isolate NM31 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
492	MT651326	Aphis craccivora isolate DELIND01 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
493	KR084997	Aphis craccivora voucher Ac1 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
494	KY840930	Aphis craccivora voucher BIOUG02527-B03 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
495	MW491803	Aphis craccivora isolate NM34 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
496	MN319880	Aphis craccivora voucher NIBGE APH-00440 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
497	KY836217	Aphis craccivora voucher BIOUG02439-H06 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
498	MN320032	Aphis craccivora voucher NIBGE APH-00343 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%
499	AB506715	Aphis craccivora mitochondrial COI gene for cytochrome oxidase subunit I, partial cds, isolate: Acra5	658	100.0%	535.0	0.00e+00	93.8%
500	MW491796	Aphis craccivora isolate NM28 cytochrome c oxidase subunit I (COX1) gene, partial cds; mitochondrial	658	100.0%	535.0	0.00e+00	93.8%

Selected alignment

Selected taxonomy

Kingdom
Phylum
Class
Order
Family
Genus
Species

Boxplot of BLAST hit identity percent grouped by genus

The boxplot above shows the identity (%) of BLAST hits grouped by genus. Each data point shows the alignment identity between the query and matched reference sequence. The analyst may wish to use this to make a subjective genus-level identification for the sample.

Analyst evaluation

Taxa of interest

This sections shows the taxa of interest specified by the submitter. Each of these taxa has been cross-referenced against the candidate species to determine if they might match the taxonomic identity of the sample. A blank row indicates a TOI that did not match any candidate species, meaning that it is unlikely that the sample matches that TOI.

Taxon of interest	Match rank	Match taxon	Match species	Match accession	Match identity
Aphis spiraecola	species	Aphis spiraecola	Aphis spiraecola	MT445566	0.998
Aphididae	family	Aphididae	Aphis spiraecola	MT445566	0.998

See the Database coverage section to see database coverage for taxa of interest.

Reference sequence source diversity

This analysis evaluates how many independent sources have contributed to reference sequences for each candidate species. This provides a measure of confidence in the taxonomic annotation of references sequences. A sequence that has been annotated by multiple independent sources is more likely to have a correct taxonomic annotation.

Analyst evaluation

No candidate species to report on.

Database coverage of target taxa

The target taxa include candidate species, the preliminary morphology ID, and any taxa of interest provided by the submitter. Each of these taxa are independently evaluated against the reference database to determine whether sufficient reference data exists to support identification of the target taxon. Insufficient coverage of a taxon can result in that taxon not be correctly identified as the taxonomic identity of the sample. For example, if the sample is Homo sapiens, but Homo sapiens sequences are not included in the reference database, the analysis will be unable to identity Homo sapiens as the correct taxonomic identity, and will most likely assign the closest relative with reference data as the taxonomic identity.

Analyst evaluation

Preliminary ID

Taxa of interest

Database coverage

Flag 5.1NA: This taxon could not be fully assessed
Reasoning: Assessment of related species is only possible for taxa at rank genus/species

Database coverage of Aphididae

Flag 5.1NA: This taxon could not be fully assessed
Reasoning: Assessment of related species is only possible for taxa at rank genus/species

NA records

There are NA sequences in the reference database for Aphididae at the given locus COI.

Global occurrence records for Aphididae.
Note that the occurrence data are not exhaustive, and it is possible for this species to occur in regions not shown on the map.

Database coverage

Flag 5.2B: The database has some support for species in this genus
Reasoning: 10-90% of related taxa have reference sequence(s) at the given locus

Database coverage of Aphis spiraecola

Flag 5.1A: The reference data supports this taxon well
Reasoning: The given locus for this taxon is well represented in reference database (>5 entries)

479 records

There are 479 sequences in the reference database for Aphis spiraecola at the given locus COI.

Global occurrence records for Aphis spiraecola.
Note that the occurrence data are not exhaustive, and it is possible for this species to occur in regions not shown on the map.

Database coverage of species in genus Aphis

Flag 5.2B: The database has some support for species in this genus
Reasoning: 10-90% of related taxa have reference sequence(s) at the given locus

/ (%) sequence records were found in the reference database for:

Species in the genus Aphis
At the target locus COI

Number of GenBank records at locus COI

Database coverage of species in genus Aphis that occur in country of origin

Flag 5.3A: Probability that a different related species from the country of origin is the true taxonomic identity: LOW
Reasoning: All species in genus from country of origin have reference sequence(s) for this locus

/ (%) sequence records were found in the reference database for:

Species in the genus Aphis
At the target locus COI
In the sample country of origin Netherlands

Number of GenBank records at locus COI

Database coverage

Flag 5.1NA: This taxon could not be fully assessed
Reasoning: Assessment of related species is only possible for taxa at rank genus/species

Database coverage of Aphididae

Flag 5.1NA: This taxon could not be fully assessed
Reasoning: Assessment of related species is only possible for taxa at rank genus/species

NA records

There are NA sequences in the reference database for Aphididae at the given locus COI.

Global occurrence records for Aphididae.
Note that the occurrence data are not exhaustive, and it is possible for this species to occur in regions not shown on the map.

Intraspecies diversity

This section provides a phylogeny of the candidate reference sequences. The analyst can use this to make a subjective observation on how well the reference sequences are able to distinguish between species. If the phylogeny shows distinct clades for each species, we can be confident that the molecular data are capable of distinguishing between those species. However, if the phylogeny shows overlap between species, this reduces the capacity of the molecular data to confidently distinguish between those species. In some cases, we may see the query sequence falling outside of the adjacent species' clades, which indicates that our query species is not represented in the reference database, which could indicate a rare or novel species.

Analyst evaluation

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The phylogetic tree was constructed with FastME using the Neighbor-Joining method. Multiple-sequence alignment of the candidate reference sequences was performed using MAFFT. The visualization is rendered with TidyTree.

Taxonomy Check

The following resources can be used to ensure that the given taxonomy is legitimate and current.

Taxa	Database
General	GBIF
General	ITIS
Mealybugs & scale	ScaleNet database
Thrips	Thripswiki
Spider Mites	Spider Mites Database
Psocodea (Barklice, Booklice, and Parasitic Lice)	Psocodea Species File Online
Orthoptera	Orthoptera Species File Online
Drosophilidae	TaxoDros
Diptera	Catalog of the Diptera of the Australasian and Oceanian Regions Systema Dipterorum
Aphids	Aphid Species File
Ants	AntWeb AntCat
Lepidoptera (butterflies and moths)	The Global Lepidoptera Names Index
Gracillariidae (primitive moths)	Global Taxonomic Database of Gracillariidae
Pyralidae (pyralid moths)	Global Information System on Pyraloidea
Tortricidae (tortrix moths)	Tortricidae Resources on the Net

Taxonomic assignment report

Sample details

Input DNA sequence

Result overview

Analyst evaluation

Identification of candidate species

BLAST search results

Analyst evaluation

Taxa of interest

Reference sequence source diversity

Analyst evaluation

Database coverage of target taxa

Analyst evaluation

Database coverage of Aphididae

Database coverage of Aphis spiraecola

Database coverage of species in genus Aphis

Per-species reference sequence counts

Database coverage of species in genus Aphis that occur in country of origin

Per-species reference sequence counts

Database coverage of Aphididae

Intraspecies diversity

Analyst evaluation

Taxonomy Check